We narrowed to 5,817 results for: pcas

1. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAX APP C60

   Plasmid

   #30152

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein C60
   

   Expression

   Mammalian

   Mutation

   fragment

   Available Since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-EGFP-C-IFT70B

   Plasmid

   #218729

   Purpose

   Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

   Insert

   IFT70B (TTC30B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-EGFP-C-IFT52

   Plasmid

   #218725

   Purpose

   Expresses N-terminally EGFP-tagged IFT52 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

   Insert

   IFT52 (IFT52 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-nDsRedFL-intron

   Plasmid

   #177804

   Purpose

   An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   intron
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mCherry-ATG14

   Plasmid

   #203567

   Purpose

   Mammalian expression of mCherry tagged ATG14

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG14 (ATG14 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-GFP-G3BP1(2)

   Plasmid

   #136059

   Purpose

   G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-sREACh-Actin

   Plasmid

   #21949

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin
   

   Expression

   Mammalian

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. hGli3 NTOTAL in AINGpCAGGS

    Plasmid

    #121973

    Purpose

    medium Gli3 transcriptional repressor

    Depositor

    James Briscoe

    Article

    Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

    Insert

    hGli3 NTOTAL (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc and Linker A IRES NLS GFP pCAGGS

    Expression

    Mammalian

    Mutation

    C terminus deletion from zinc finger domain

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Neo-PuroDTK

    Plasmid

    #84036

    Purpose

    PiggyBac cassette contains Neomycin, puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Promoter

    CAG promoter

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG MCP-VP64

    Plasmid

    #92357

    Purpose

    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    MCP-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-GFP-G3BP1(1)

    Plasmid

    #136058

    Purpose

    G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-GST FNIP2

    Plasmid

    #164982

    Purpose

    FNIP2 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FNIP2 (FNIP2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-rTetRDBD-GBP1

    Plasmid

    #50791

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    rTetR DNA binding domain-GBP1 fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-H2B-GFP

    Plasmid

    #184777

    Purpose

    Expresses H2B-GFP in human pluripotent stem cells

    Depositor

    Ivana Barbaric

    Article

    Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

    Insert

    H2BC11 (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-AaCas12b-2AeGFP

    Plasmid

    #121945

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GST-ATG14

    Plasmid

    #203566

    Purpose

    Mammalian expression of GST tagged ATG14

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG14 (ATG14 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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