We narrowed to 1,598 results for: pfas

1. pMaCTag-13

   Plasmid

   #119992

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry
   

   Use

   Pcr template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JAK3 (JAK3A-c023)

   Plasmid

   #98229

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JAK3A (JAK3 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Expression

   Mutation

   pfam00069:Pkinase 822-1091

   Promoter

   polyhedrin

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFM571

   Plasmid

   #74634

   Purpose

   alias pFA6a-mCherry-kanMX6 - vector for C-terminal tagging of yeast genes with mCherry and G418 selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMH062

   Plasmid

   #225537

   Purpose

   Expresses IS621 in insect cells

   Depositor

   Hiroshi Nishimasu

   Article

   Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

   Insert

   IS621
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIM691

   Plasmid

   #74641

   Purpose

   alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   ymTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFBNb-cALFA

   Plasmid

   #159990

   Purpose

   For the protein expression with ALFA tag at C-terminus in insect cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-EGFP-T2A-ACREB-WPRE

   Plasmid

   #40867

   Depositor

   Harold Gainer

   Article

   Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

   Insert

   ACREB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-07

   Plasmid

   #124786

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIM573

   Plasmid

   #74637

   Purpose

   alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   ymTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDest-623 (attR4-attR3)

    Plasmid

    #161878

    Purpose

    Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMaCTag-23

    Plasmid

    #120002

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL70008

    Plasmid

    #50336

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIM700

    Plasmid

    #74639

    Purpose

    alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-P31

    Plasmid

    #120042

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    V5
    

    Use

    Pcr template

    Tags

    V5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-12

    Plasmid

    #119991

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIM690

    Plasmid

    #74638

    Purpose

    alias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BRD7 (BRD7A-c005)

    Plasmid

    #98245

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD7A (BRD7 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00439:Bromodomain 136-223

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-15

    Plasmid

    #119994

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCoofy63

    Plasmid

    #122015

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge