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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98-ires-GFP

Plasmid

#237481

Purpose

Express NUP98 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98 (NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98

Plasmid

#237482

Purpose

Express HA-tagged NUP98 in dual promoter lentiviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98 (NUP98 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.1

Plasmid

#172319

Purpose

expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.1 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

Promoter

CMV

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Mu

Plasmid

#177578

Purpose

expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Mu variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Lambda

Plasmid

#177579

Purpose

expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Lambda variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI

Plasmid

#160480

Purpose

Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475RG502R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine, Alanine 475 to A…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI

Plasmid

#160479

Purpose

Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RG496R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Glyci…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI

Plasmid

#160478

Purpose

Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-L455RA475R

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

changed Leucine 455 to Arginine and changed Alani…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS U6 WIP RNAi

Plasmid

#11774

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP RNAi (WIPF1 Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
CL20_MSCV-NUP98::KDM5A-ires-GFP

Plasmid

#237489

Purpose

Express NUP98::KDM5A in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::KDM5A (NUP98, KDM5A Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::LNP1-ires-GFP

Plasmid

#237494

Purpose

Express NUP98::LNP1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::LNP1 (LNP1, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::HOXA9-ires-GFP

Plasmid

#237484

Purpose

Express NUP98::HOXA9 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::HOXA9 (HOXA9, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::SETBP1

Plasmid

#237491

Purpose

Express HA-tagged NUP98::SETBP1 in dual promoter lentiviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::SETBP1 (NUP98, SETBP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::NSD1-ires-GFP

Plasmid

#237483

Purpose

Express NUP98::NSD1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::NSD1 (NUP98, NSD1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::NSD1

Plasmid

#237497

Purpose

Express HA-tagged NUP98::NSD1 in dual promoter lentiviral vector

Depositor

Jeffery Klco

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::NSD1 (NUP98, NSD1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::HOXA9

Plasmid

#237498

Purpose

Express HA-tagged NUP98::HOXA9 in dual promoter lentiviral vector

Depositor

Jeffery Klco

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::HOXA9 (NUP98, HOXA9 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::KDM5A

Plasmid

#237499

Purpose

Express HA-tagged NUP98::KDM5A in dual promoter lentiviral vector

Depositor

Jeffery Klco

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::KDM5A (NUP98, KDM5A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge