We narrowed to 7,674 results for: ski

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3-del5

Plasmid

#44732

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Mutation

The first 450 nt were deleted from the MEG3 cDNA.

Promoter

hCMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pMC-J6-NbGP41-sfGFP-VP64-GB1-J9

Plasmid

#202007

Purpose

MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

NbGP41-sfGFP-VP64-GB1

Use

Synthetic Biology; Moclo cloning vector

Tags

GB1 and sfGFP

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302 MX1-V5 puro

Plasmid

#158640

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged MX1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

Mx1 (MX1 Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9

Plasmid

#89995

Purpose

Expresses high levels of WT Cas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

hCas9

Tags

3xFlag, SV40 NLS, and T2A peptide

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-H

Plasmid

#222846

Purpose

Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-H

Expression

Yeast

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-L

Plasmid

#222847

Purpose

Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Expression

Yeast

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MPHOSPH8 ts2

Plasmid

#115875

Purpose

MPHOSPH8 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MPHOSPH8 shRNA (MPHOSPH8 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHP138 Myc-TRF2-deltaB deltaM

Plasmid

#164255

Purpose

for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TRF2

Tags

Myc

Expression

Mammalian

Mutation

deletion of amino acids-86 and 496-542

Promoter

Tetracycline inducible promotor

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts1

Plasmid

#115862

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS--GFP-p300

Plasmid

#183925

Purpose

p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-p300

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pscALPS- HIV2-ROD vpx

Plasmid

#115816

Purpose

Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV2ROD Vpx (M15390)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts3

Plasmid

#115864

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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ClCry4 Y319D

Plasmid

#160649

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 PHR Y319D

Tags

Hexa his

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-H-V5-His

Plasmid

#222850

Purpose

E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-H

Tags

V5-His

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only