We narrowed to 13,073 results for: BASE
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Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectPromoterAe. aegypti PUb and endogenous AGO1Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187916PurposeExpresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
Tags3xFLAGExpressionMammalianPromoterCMV PromoterAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PYL1_nTEVp
Plasmid#119213PurposeExpresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nTEVp in sufion with PYL1
TagsAU1 tagExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-8his
Plasmid#111847PurposeMammalian expression plasmid for soluble BG505 SOSIP.664DepositorInsertHIV-1 Env (BG505 SOSIP.664)
Tags8his purification tag and CD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H3C2
Plasmid#207780PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.DepositorAvailable SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
TagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastPromoterTEFAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
Tags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABI_cTEVp
Plasmid#119214PurposeExpresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domainDepositorInsertsplit cTEVp in fusion with ABI
TagsMyc tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only