We narrowed to 851 results for: gfp cassette

1. pRSET a sfGCaMP6s

   Plasmid

   #100022

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ:F3-CAGGS GPHTK-F

   Plasmid

   #112666

   Purpose

   Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   eGFP
   

   Use

   AAV; Geme targeting vector for integration at the…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2Lox

   Plasmid

   #34635

   Depositor

   Michael Kyba

   Article

   Iacovino et al Stem Cells. 2011 Oct;29(10):1580-8.

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PGK promoter

   Available since

   Feb. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pTSTEP

   Plasmid

   #72334

   Purpose

   Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

   Depositor

   Avital Rodal

   Article

   Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

   Insert

   RRS-TagRFPT-p10-[Golic+] RRS-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK0_003

   Plasmid

   #123925

   Purpose

   Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cas9-sgRNA destination
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET a sfGCaMP6s-T78H

   Plasmid

   #100023

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s-T78H
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP and T78H m…

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET a sfMatryoshCaMP6s

   Plasmid

   #100020

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfMatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGH42

   Plasmid

   #107249

   Purpose

   Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   vipp1-gfp
   

   Use

   Suicide vector for destined for double homologous…

   Tags

   mGFPmut3

   Expression

   Bacterial

   Mutation

   Promoter

   native promoter of sll0617

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PAX6 donor plasmid

    Plasmid

    #105239

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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