-
Plasmid#84735PurposeSimultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterFMV/35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only
-
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcRfaHKOW-L1L2NusG
Plasmid#113126PurposeExpresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residuesDepositorInsertRfaH (rfaH Escherichia coli)
UseTagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 1-107, replaced L1 and L2 loops …PromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro_FlnaABD-TS-16flag
Plasmid#234868PurposeExpresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.DepositorInsertFlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
UseLentiviralTagsGFP, RFP, FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro_FlnaABD-15-TS-16-24flag
Plasmid#234869PurposeExpresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.DepositorInsertFlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
UseLentiviralTagsGFP, RFP, FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
-
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_WT
Plasmid#98662PurposeExpresses MBP-tagged full length hnRNPA2DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfrbeta-EGFPN
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRbeta (Pdgfrb Mouse)
UseTagsC-term EGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only