We narrowed to 161,982 results for: addgene

1. LRG2.1-BFP

   Plasmid

   #120577

   Purpose

   Lentiviral plasmid to co-express a guide RNA and EBFP.

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HO-Poly-KanMX4-HO

   Plasmid

   #51662

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACRISPR-KM

   Plasmid

   #203587

   Purpose

   A sgRNA expression plasmid for genome editing in Burkholderia

   Depositor

   Leonilde Moreira

   Article

   Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

   Plasmid

   #159911

   Purpose

   Mutagenesis of Kcnq3

   Depositor

   Larry Zweifel

   Insert

   Kcnq3 (Kcnq3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACRISPR-CM

   Plasmid

   #203588

   Purpose

   A sgRNA expression plasmid for genome editing in Burkholderia

   Depositor

   Leonilde Moreira

   Article

   Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. KLHL40 (KBTBD5A-c006)

   Plasmid

   #98233

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KBTBD5A (KLHL40 Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAPK3

   Plasmid

   #73235

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 42-330

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-G9a-Hygro

   Plasmid

   #104408

   Purpose

   transient expression of dCas9-G9a fusion protein

   Depositor

   Stephan Beck

   Insert

   G9a (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118158

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB_EF1a_coGag

    Plasmid

    #212656

    Purpose

    piggyBac vector to express codon optimized MLV Gag

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSANG14-3F

    Plasmid

    #39265

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Tags

    6xHis, Alkaline phosphatase, pelB leader, and tri…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4IKP

    Plasmid

    #60080

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CARM1:JMC049-A06:C226836 (CARM1 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Expression

    Mutation

    See comments

    Promoter

    polyhedrin

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 5TEY (METTL3)

    Plasmid

    #101892

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL3 (METTL3 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis, TEV

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pPB_EF1a_FLAG-tNGFR_ZeoR

    Plasmid

    #212649

    Purpose

    piggyBac vector to express FLAG-tNGFR with zeocin resistance

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    tNGFR
    

    Use

    Piggybac

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACRISPR-TP

    Plasmid

    #203589

    Purpose

    A sgRNA expression plasmid for genome editing in Burkholderia

    Depositor

    Leonilde Moreira

    Article

    Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CFL1 (CFL1A-c002)

    Plasmid

    #98237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CFL1A (CFL1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00241:Cofilin_ADF 27-148

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACλCI

    Plasmid

    #53730

    Purpose

    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    bacteriophage lambda CI protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHdCas9

    Plasmid

    #137080

    Purpose

    Expression of Himar-dCas9 in bacteria

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-H1-GFP-neomycin

    Plasmid

    #37632

    Depositor

    Valeri Vasioukhin

    Article

    Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only