We narrowed to 4,425 results for: Pet2;
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Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis
Plasmid#246980PurposeExpresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.DepositorInsertFLP Recombinase
UseSynthetic BiologyTags8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrepExpressionBacterialPromoterT7Available SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-GFP-UROD-N367A
Plasmid#236742PurposeProtein overexpression of eGFP-human uroporphyrinogen decarboxylase N367ADepositorInserteGFP-Uroporphyrinogen decarboxylase human N367A (UROD Human, Synthetic)
TagsHis6 and eGFPExpressionBacterialMutationN367AAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(66-140) K96A/K97A
Plasmid#240590PurposeOverexpress truncated alpha-Synuclein proteinDepositorInsertaSyn (SNCA Human)
Tags6xHisExpressionBacterialMutationaa 66-140 only, K96A, K97APromoterT7Available SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(66-140) E104A/E105A
Plasmid#240589PurposeOverexpress truncated alpha-Synuclein proteinDepositorInsertaSyn (SNCA Human)
Tags6xHisExpressionBacterialMutationaa 66-140 only, E104A, E105APromoterT7Available SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(66-140) E83K/K97E
Plasmid#240591PurposeOverexpress truncated alpha-Synuclein proteinDepositorInsertaSyn (SNCA Human)
Tags6xHisExpressionBacterialMutationaa 66-140 only, E83K, K97EPromoterT7Available SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(66-140) K80E/E83K
Plasmid#240593PurposeOverexpress truncated alpha-Synuclein proteinDepositorInsertaSyn (SNCA Human)
Tags6xHisExpressionBacterialMutationaa 66-140 only, E83K, K80EPromoterT7Available SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(66-140) E83K/K96E
Plasmid#240592PurposeOverexpress truncated alpha-Synuclein proteinDepositorInsertaSyn (SNCA Human)
Tags6xHisExpressionBacterialMutationaa 66-140 only, E83K, K96EPromoterT7Available SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS
Plasmid#224697PurposeBacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tagDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
TagsGly-Gly-6xHisExpressionBacterialMutationsilent mutation C to T at position 10546 (elimina…PromoterT7Available SinceJuly 2, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS
Plasmid#224698PurposeBacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tagDepositorInsertSARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
TagsGly-Gly-6xHisExpressionBacterialMutationsilent mutation C to T at position 10546 (elimina…PromoterT7Available SinceJuly 2, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ECMAB - pET22b Ag43 160N 6H Mms6
Plasmid#225539PurposeConstruction of extracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Autotransporter protein
TagsnoExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H mCherry-R5 AmpR
Plasmid#225141PurposeRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 160N, mCherry
TagsAg43 protein fused to silaffin-R5 and mCherryExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiA-FLAG
Plasmid#229220PurposeFor expression of T. elongatus KaiA proteinDepositorAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiB-FLAG
Plasmid#229221PurposeFor expression of T. elongatus KaiB proteinDepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-SeKaiB-K25C-FLAG
Plasmid#229225PurposeFor expression of S. elongatus KaiB protein mutantDepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5
Plasmid#226352PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
Plasmid#226353PurposeExpression of titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
Tags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
Tags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Cdc42/13C-FKBP-MBP
Plasmid#214291PurposeExpress split-Cdc42 fragment fused with FKBP and MBPDepositorInsertSplit-Cdc42 fragment fused with FKBP (CDC42 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Rac1/13C-FKBP-MBP
Plasmid#214295PurposeExpress split-Rac1 fragment fused with FKBP and MBPDepositorInsertSplit-Rac1 fragment fused with FKBP (RAC1 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
Tags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only