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Showing: 1831 - 1860 of 6111 results

  1. KBB528

    Plasmid
    #185104
    Purpose
    C-terminal truncation of Pom152 fused to GFP
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    POM152(1-???)-HA-GFP
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185104-plasmid-map-sequence-id-368165

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  2. ADH1pro_GCR1_SIR4

    Plasmid
    #138618
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR1 (GCR1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138618-plasmid-map-sequence-id-274926

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  3. ADH1pro_CBF1_SIR4

    Plasmid
    #138616
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    CBF1 (CBF1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138616-plasmid-map-sequence-id-275117

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  5. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  6. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  7. Fyv6-NFLAG del103-173

    Plasmid
    #234134
    Purpose
    Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234134-plasmid-map-sequence-id-466524

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  8. Fyv6-NFLAG del134-173

    Plasmid
    #234135
    Purpose
    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234135-plasmid-map-sequence-id-466521

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  9. pUC57-T7-PAB1-NLuc

    Plasmid
    #219984
    Purpose
    PAB1-5'UTR-NLuc mRNA synthesis
    Depositor
    Simon Alberti
    Insert
    PAB1-5'UTR-NLuc (PAB1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219984-plasmid-map-sequence-id-436830

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  10. pOCC121-MBP-monoGFP-eIF4G

    Plasmid
    #219972
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis and MBP-monoGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219972-plasmid-map-sequence-id-436768

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  11. LDB361

    Plasmid
    #185435
    Purpose
    Bacterial expression of full length NUP2 as fusion with GST
    Depositor
    Laura Davis, Ken Belanger
    Insert
    NUP2
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185435-plasmid-map-sequence-id-367560

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  12. pROS15_MATz

    Plasmid
    #166100
    Purpose
    Expression of dual gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166100-plasmid-map-sequence-id-328822

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  13. pUPD2 CUP2 (GB2716)

    Plasmid
    #160599
    Purpose
    Copper dependent transcription factor
    Depositor
    Diego Orzaez
    Insert
    CUP2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160599-plasmid-map-sequence-id-322067

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  14. LHP428-YCplac33-STE2-378Stop

    Plasmid
    #32172
    Depositor
    Linda Hicke
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Stop codon introduced at aa 378
    Promoter
    STE2
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. LHP1048 - pFB-RSP5

    Plasmid
    #32434
    Depositor
    Linda Hicke
    Insert
    RSP5 (RSP5 Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P-P10 and P-Pol
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. KBB609

    Plasmid
    #185110
    Purpose
    Pom152 N-terminus in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 N-term-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185110-plasmid-map-sequence-id-368108

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  17. pGAL-SKI1-NAT

    Plasmid
    #160382
    Purpose
    Overxpression of SKI1/XRN1 in yeast
    Depositor
    John McCusker
    Insert
    SKI1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160382-plasmid-map-sequence-id-314520

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  18. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid
    #58836
    Purpose
    Expresses GFP-Tagged Opi1 + Terminator in yeast
    Depositor
    Tim Levine
    Insert
    Opi1p (OPI1 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Available since
    Sept. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. LHP462 - Ub-HA

    Plasmid
    #32169
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pOCC240-MBP-Pab1-mCherry

    Plasmid
    #219980
    Purpose
    Pab1p-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    Pab1p-mCherry (PAB1 Budding Yeast)
    Use
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219980-plasmid-map-sequence-id-436810

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  21. pOCC240-MBP-eIF4A-mCherry

    Plasmid
    #219982
    Purpose
    eIF4A-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4A-mCherry (TIF1 Budding Yeast)
    Use
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219982-plasmid-map-sequence-id-436882

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  22. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Matthias Heinemann
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166111-plasmid-map-sequence-id-330321

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  23. pMLLCK-J72038

    Plasmid
    #26183
    Depositor
    Christopher Anderson
    Insert
    Med21 aa1-132 for N-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26183-plasmid-map-sequence-id-12304

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  24. pMLLKA-J72039

    Plasmid
    #26184
    Depositor
    Christopher Anderson
    Insert
    Med7 aa102-205 for C-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26184-plasmid-map-sequence-id-12305

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  25. pMLLKA-J72040

    Plasmid
    #26185
    Depositor
    Christopher Anderson
    Insert
    Med21 aa1-132 for C-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only

  26. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  27. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  28. pPICZac-STE2-1D4

    Plasmid
    #133437
    Purpose
    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133437-plasmid-map-sequence-id-267729

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  29. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  30. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 1831 - 1860 of 6111 results