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EFSp-GFP-YAP (S94A)

Plasmid

#174169

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

EFS

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PGKp-GFP-YAP

Plasmid

#174172

Purpose

Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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sgYAP-TAZ-3

Plasmid

#229453

Purpose

knockout of YAP1 and WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-TAZ-3 (YAP1, WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PGKp-GFP-YAP (S94A)

Plasmid

#174173

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLViN-iRFP670-α-cateninA+ΔβH

Plasmid

#229707

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-TAZ-2

Plasmid

#229452

Purpose

knockout of YAP1 and WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-TAZ-2 (YAP1, WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EFSp-GFP-YAP (5SA)

Plasmid

#174170

Purpose

Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Five LATS phosphorylation sites (S61, S109, S127,…

Promoter

EFS

Available since

Sept. 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PGKp-GFP-YAP (5SA)

Plasmid

#174174

Purpose

Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Five LATS phosphorylation sites (S61, S109, S127,…

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-MAGI1

Plasmid

#23523

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEMS1369

Plasmid

#29170

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple105 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1368

Plasmid

#29169

Purpose

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple104 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1086

Plasmid

#29020

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple106 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1084

Plasmid

#29019

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple104 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1370

Plasmid

#29171

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple106 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1367

Plasmid

#29168

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple103 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1083

Plasmid

#29018

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple103 (HAP1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_JUN_WT

Plasmid

#82138

Purpose

Gateway Donor vector containing JUN , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JUN (JUN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pIG-755_HA-GD2-28z_CAR_RFP-JUN

Plasmid

#207494

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-JUN, HA-GD2-28z_CAR (JUN Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge