We narrowed to 1,246 results for: LIPI

1. Raichu-OsRac1 WT

   Plasmid

   #133262

   Purpose

   Biosensor for monitoring activation for rice small GTPase OsRac1

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1 (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEE032

   Plasmid

   #176789

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   401
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUMO ACD11

   Plasmid

   #170738

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

   Insert

   acd11 (ACD11 Mustard Weed)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRPML1-G-GECO1.2-ERES

   Plasmid

   #207144

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Mcoln1 (MCOLN1 Human)
   

   Use

   Tags

   ER-Export Signal (ERES) and G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

   Plasmid

   #212112

   Purpose

   Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of GABARAP

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO CPTP

   Plasmid

   #170735

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   CPTP (CPTP Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFLAG R106L-CPTP

   Plasmid

   #170744

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   CPTP (CPTP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R106L

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG K60A-CPTP

    Plasmid

    #170743

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K60A

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

    Plasmid

    #212113

    Purpose

    Expression of GABARAP split tandem protein with mCherry

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mCherry-GABARAP (GABARAP Human, Discosoma sp.)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of upstream GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSUMO R106L-CPTP

    Plasmid

    #170737

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    R106L-CPTP (CPTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    R106L

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUMO K60A-CPTP

    Plasmid

    #170736

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    K60A-CPTP (CPTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    K60A

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBbi-Pur-Lyn-AktAR2

    Plasmid

    #125197

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    Lyn-AktAR2
    

    Use

    Transposon

    Tags

    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRPML1-mCherry

    Plasmid

    #135189

    Purpose

    Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TRPML1 (Mcoln1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLOC-MCOLN1

    Plasmid

    #131583

    Purpose

    For expression of human MCOLN1 in mammalian cells, GFP-tagged

    Depositor

    Sean Morrison

    Article

    Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

    Insert

    MCOLN1 (MCOLN1 Human)
    

    Use

    Lentiviral

    Tags

    turboGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP

    Plasmid

    #212106

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of upstream GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSBbi-Pur-Lyn-AktAR2-D

    Plasmid

    #125198

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    Lyn-AktAR2-D
    

    Use

    Transposon

    Tags

    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

    Plasmid

    #112762

    Purpose

    express GFP-4EBP1-Rheb15(CAAX mutation)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Rheb15(CAAX mutant)

    Expression

    Mutation

    CAAX mutation to prevent lipid localization

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid

    #169168

    Purpose

    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    6X Histidine Tag, TEV cleavage site, mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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