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LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-hnRNP-H

Plasmid

#23020

Depositor

Douglas Black

Article

Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

Insert

hnRNP-H (HNRNPH1 Human)

Tags

His

Expression

Bacterial

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Article

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human)

Tags

DsRed

Expression

Bacterial and Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BlastR

Plasmid

#194059

Purpose

Mammalian expression of evoCjCas9 nuclease

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPAICS-EGFP

Plasmid

#99108

Purpose

Expresses human PAICS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PAICS (PAICS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-ABE8e_gRNA

Plasmid

#202559

Purpose

P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-LOC339287(MSL1)

Plasmid

#29762

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL1 (MSL1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_TOM22

Plasmid

#111697

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TOM22 (TOMM22 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBA1A

Plasmid

#111695

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBA1A (TUBA1A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-TadCDd-BlastR

Plasmid

#202558

Purpose

Mammalian expression of evoCjCas9 TadCDd

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-MSL3L1

Plasmid

#29761

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL3L1 (MSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
MAC_C_PDK1

Plasmid

#111672

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

PDK1 (PDK1 Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_SCO1

Plasmid

#111675

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

SCO1 (SCO1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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