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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,788 results for: golden gate

Showing: 1221 - 1240 of 3788 results

  1. P58m

    Plasmid
    #120950
    Purpose
    MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLas Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120950-plasmid-map-sequence-id-235962

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  2. P59m

    Plasmid
    #120951
    Purpose
    MoClo golden gate assembly AB part for pLux76B (Adam Meyer's improved pLux promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLux Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120951-plasmid-map-sequence-id-236155

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  3. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

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  4. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  5. pAIP4_9C74

    Plasmid
    #234238
    Purpose
    Heterologous protein expression of variant of super folded GFP in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    9C74
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234238-plasmid-map-sequence-id-464202

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  6. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  7. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  8. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  9. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  10. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

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  11. pKS1603

    Plasmid
    #239527
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239527-plasmid-map-sequence-id-479035

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  12. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

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  13. pKS1615

    Plasmid
    #239546
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239546-plasmid-map-sequence-id-478363

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  14. pLII-CRISPR for CKA4

    Plasmid
    #238521
    Purpose
    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha4 (AT2G23070 Mustard Weed)
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238521-plasmid-map-sequence-id-477173

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  15. pKS1613

    Plasmid
    #239531
    Purpose
    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239531-plasmid-map-sequence-id-475886

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  16. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

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  17. pAIP4_1ELK

    Plasmid
    #234217
    Purpose
    Heterologous protein expression of target of Myb protein 1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ELK (TOM1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234217-plasmid-map-sequence-id-464182

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  18. pAIP4_5XBC

    Plasmid
    #234213
    Purpose
    Heterologous protein expression of 3C-like proteinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5XBC
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234213-plasmid-map-sequence-id-464178

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  19. pAIP4_3HNX

    Plasmid
    #234199
    Purpose
    Heterologous protein expression of cyanovirin-N homolog (CV-N homolog) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3HNX
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234199-plasmid-map-sequence-id-464164

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  20. pAIP4_4A02

    Plasmid
    #234194
    Purpose
    Heterologous protein expression of lytic chitin monooxygenase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4A02
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234194-plasmid-map-sequence-id-464159

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Showing: 1221 - 1240 of 3788 results