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pLX_TRC311 ETV1

Plasmid

#74981

Purpose

Constitutive ETV1 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Nucleocapsid

Plasmid

#168906

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Nucleocapsid (N )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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YES1 gRNA (BRDN0001148961)

Plasmid

#77967

Purpose

3rd generation lentiviral gRNA plasmid targeting human YES1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

YES1 (YES1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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YES1 gRNA (BRDN0001145317)

Plasmid

#77965

Purpose

3rd generation lentiviral gRNA plasmid targeting human YES1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

YES1 (YES1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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YES1 gRNA (BRDN0001487120)

Plasmid

#77966

Purpose

3rd generation lentiviral gRNA plasmid targeting human YES1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

YES1 (YES1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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UBE2LB_pLX307

Plasmid

#98380

Purpose

Lentiviral expression of UBE2L6

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

UBE2L6 (UBE2L6 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001162487)

Plasmid

#76139

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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