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pcDNA 3.1 SIRT1-deltaExon2

Plasmid

#105671

Purpose

Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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p21-S208C/S242R/M694V-MEFV

Plasmid

#134706

Purpose

Lentiviral expression of human MEFV S208C/S242R/M694V after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

p.S208C/S242R/M694V

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

Plasmid

#170200

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Promoter

CMV51

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

Plasmid

#112762

Purpose

express GFP-4EBP1-Rheb15(CAAX mutation)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)

Use

Lentiviral

Tags

Rheb15(CAAX mutant)

Expression

Mutation

CAAX mutation to prevent lipid localization

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Expression

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

Plasmid

#72608

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine37 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Pink1 [N4/15-2b] - Chimeric

Plasmid

#227032

Purpose

Mammalian expression plasmid of anti-Pink1 (Human). Derived from hybridoma N4/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Pink1 (Homo sapiens) recombinant mouse monoclonal antibody. (PINK1 Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/26R]

Plasmid

#225386

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 [L51/73R]

Plasmid

#225373

Purpose

Mammalian Expression Plasmid of anti-MMP9 (Rat) IgG2a R-mAb. Derived from hybridoma L51/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-MMP9 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Mmp9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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