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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Expression

Mammalian

Promoter

U1A

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFD_L

Plasmid

#198276

Purpose

For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_L

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2A_tdTomato

Plasmid

#184045

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-tTA

Plasmid

#162598

Purpose

human CD81-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHD157

Plasmid

#84033

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and fabp10

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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