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Dcp1 82-129-EGFP

Plasmid

#201427

Purpose

Bacterial protein expression of Dcp1 82-129

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 82-129 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1 1-240-EGFP

Plasmid

#201435

Purpose

Bacterial protein expression of Pat1 1-240

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-240 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3 67-282-EGFP

Plasmid

#201433

Purpose

Bacterial protein expression of Edc3 67-282

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 67-282 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1 241-422-EGFP

Plasmid

#201436

Purpose

Bacterial protein expression of Pat1 241-422

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 241-422 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2 1-300-EGFP

Plasmid

#201428

Purpose

Bacterial protein expression of Dcp2 1-300

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-300 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1 423-796-EGFP

Plasmid

#201437

Purpose

Bacterial protein expression of Pat1 423-796

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 423-796 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Edc3 283-551-EGFP

Plasmid

#201434

Purpose

Bacterial protein expression of Edc3 283-551

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 283-551 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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EcNusGNTD

Plasmid

#113123

Purpose

Expresses N-terminus of E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 120-181

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.15

Plasmid

#131373

Purpose

expression of MBP:PAA-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

paa-1 (paa-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.13

Plasmid

#131375

Purpose

expression of MBP:PPTR-2 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-2 (pptr-2 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.12

Plasmid

#131374

Purpose

expression of MBP:PPTR-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pptr-1 (pptr-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAL829

Plasmid

#119697

Purpose

precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C

Use

Tags

none

Expression

Bacterial

Mutation

mutations Y283D, G289C and V347C

Promoter

T5

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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R2Ct (1-520)

Plasmid

#115649

Purpose

expression of truncated Rag2 including the C-terminal region

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (1-520) (Rag2 Mouse)

Use

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag2 (aa 1-520)

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDuBir-Lbu-dCas13a-avitag

Plasmid

#100817

Purpose

Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

Depositor

Mitchell O'Connell

Article

Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

Insert

Lbu_Cas13a_R472A_H477A_R1048A_H1053A

Use

Tags

AviTag and His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Use

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge