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pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

Plasmid

#185489

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Raul Perez-Jimenez, Benjamin Kleinstiver

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

Plasmid

#185492

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Raul Perez-Jimenez, Benjamin Kleinstiver

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

D10A

Promoter

CMV and T7

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMSOX17FNV

Plasmid

#206382

Purpose

Expresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

OCT4, KLF4, c-MYC, SOX17FNV (POU5F1, KLF4, MYC, SOX17 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

Plasmid

#184549

Purpose

Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Retroviral

Tags

3xFLAG and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUltra-MDH1-ME1

Plasmid

#184465

Purpose

Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydrogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Lentiviral

Tags

fused to T2A

Expression

Mammalian

Mutation

deletion of stop codon

Promoter

UbC

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pR2HYG

Plasmid

#30170

Depositor

Lalita Ramakrishnan

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

dsRed2

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBacMam8_N_Flag_3C

Plasmid

#178818

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

Flag, 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam3_N_His8_eGFP_3C

Plasmid

#178807

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

His8, eGFP, 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacMam4_N_Flag_His8_eGFP_TEV

Plasmid

#178808

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

Flag, His8, eGFP, TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacMam7_N_Flag_TEV

Plasmid

#178817

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

Flag, TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacMam2_N_His8_eGFP_TEV

Plasmid

#178806

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

His8, eGFP, TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam5_N_Flag_His8_eGFP_3C

Plasmid

#178816

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

Flag, His8, eGFP, 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam6_C_3C_His8_Stop

Plasmid

#178810

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

3C, His8, Stop codon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGBK CUP1 Empty

Plasmid

#169710

Purpose

Yeast two-hybrid vector using a copper inducible CUP1 promoter

Depositor

Bruce Hamilton

Article

Hernandez et al bioRxiv

Type

Empty backbone

Use

Tags

GAL4 DNA Binding Domain and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

CUP1

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Z-MATR3-4

Plasmid

#166558

Purpose

scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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A-WDR5-1

Plasmid

#166515

Purpose

scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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J-CDAN1A-5

Plasmid

#166522

Purpose

scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Z-OPTN-10

Plasmid

#166562

Purpose

scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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O-TREM2-2

Plasmid

#166545

Purpose

scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Q-ACACAA-10

Plasmid

#166546

Purpose

scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Mutation

Promoter

LacZ

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge