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Plasmid#24997DepositorInsertIrx5 (Irx5 Mouse)
UseTagsHAExpressionMammalianMutationDeletes entire HD (aa 112-177)PromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex HA (P#1707)
Plasmid#14592DepositorInsertLexA
UseTagsHA and UbiquitinExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
p218 pCMV-CREM-L
Plasmid#8396DepositorInsertCREM-L
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, single LOX 511 sitePromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 E7K HA
Plasmid#8955DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationE7K: point mutation in LxCxE motif, LxCxkPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV9-CMV-eGFP
Plasmid#230928PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
p212 pCMV-EGFP/RFP
Plasmid#8389DepositorInsertfloxed EGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1
Plasmid#223315PurposeLentiviral vector expressing human PNPT1DepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-CMV-bArrestin2-TEV
Plasmid#107245PurposeVector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.DepositorInsertARRB2
UseTagsTEV(NIA), tobacco etch virus NIAExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PuroR-T2A-eGFP
Plasmid#234734PurposePuromycin resistance/eGFP cassette in mammalian cells (transfection marker)DepositorInsertPuroR-T2A-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-EGFP-NLS
Plasmid#165441PurposeExpresses nuclear EGFP from CMV promoterDepositorInsertEGFP-NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV2-CMV-eGFP
Plasmid#230930PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro_FlnaABD-TS-16flag
Plasmid#234868PurposeExpresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.DepositorInsertFlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
UseLentiviralTagsGFP, RFP, FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro_FlnaABD-15-TS-16-24flag
Plasmid#234869PurposeExpresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.DepositorInsertFlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
UseLentiviralTagsGFP, RFP, FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV flag ERR alpha
Plasmid#10975DepositorInsertERR alpha (ESRRA Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-z ONE-GO
Plasmid#189734PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#236407PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK381 (AAVS1 CMV-OsTIR1F74G)
Plasmid#140536PurposeAAVS1 CMV-OsTIR1(F74G)DepositorInsertCMV-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
UseTags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV BiP-Myc-KDEL-wt
Plasmid#27164PurposeMammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequenceDepositorInsertBiP (HSPA5 Human)
UseTagsKDEL and MycExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
UseTagsExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV FKBP-V5-sTurboID (N)
Plasmid#153002PurposeExpresses low-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA-CMV-GFP
Plasmid#85451PurposeExpress sgRNA in mammalian cellsDepositorInsertpCMV-EGFP
UseAAVTagsExpressionMutationPromoterpCMV-EGFPAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABCB10-ChR2-eYFP
Plasmid#206037PurposeExpress ChR2 in mitochondriaDepositorInsertChR2
UseTagsABCB10 mitochondrial leading sequence and EYFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA_N-mouse Bcl6
Plasmid#138410PurposeExpress mouse Bcl6 in mammalian cellsDepositorInsertB cell lymphoma 6 (Bcl6 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s ONE-GO
Plasmid#189731PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only