We narrowed to 8,143 results for: 11
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Plasmid#70089PurposeExpression of human HOXB13 from CMV promoter, puromycin selection markerDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP
Plasmid#55646PurposeCre-on/Flp-off ChR2-EYFP under the Synapsin promoterDepositorInsertChR2(H134R)-EYFP
UseAAV; Cre on/flp off chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
FpG5
Plasmid#69443Purposelentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAsDepositorInsertsminimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
UseLentiviralExpressionMammalianAvailable SinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF4Ag NanoLuc (ATG-42)
Plasmid#137777PurposeExpresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)DepositorInsertNanoLuc (Nluc)
ExpressionBacterial and MammalianMutationNanoLuc contains 16 amino acid changes compared t…PromoterT7 and CMVAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)
Plasmid#114070PurposeT7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tagDepositorInsertbacteria codon optimized LbCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRExpressionMammalianAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(WT)-mCherry
Plasmid#79422PurposeExpresses C-terminally mCherry-tagged Arf6(WT) in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
Plasmid#192151PurposeT cell CRISPR AAV-SB vectorDepositorTypeEmpty backboneUseAAVExpressionMammalianMutationNAAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-rc[Chronos-GFP]
Plasmid#59056PurposeExpression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)DepositorInsertChronos-GFP
TagsGFPExpressionMammalianPromoterCAGAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP
Plasmid#55643PurposevCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Vcre-dependent chr2-eyfpTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.3.1.ADRB2-NP
Plasmid#134376PurposeNanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.DepositorInsertADRB2-NP (ADRB2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralExpressionMammalianPromoterUBQ and SV40Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0118
Plasmid#141525PurposeLentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Dre
Plasmid#55633PurposeExpresses Dre in Mammalian CellsDepositorInsertsmCherry
Dre
UseAAVExpressionMammalianPromoterEf1a and Ef1a/IRESAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only