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pET19b_4CL:P7SHb + STS:P8SHb

Plasmid

#139793

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P7SHb
STS:P8SHb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P7S + STS:P8S

Plasmid

#139794

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P7S
STS:P8S

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Expression

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#71584

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

Depositor

T. J. Kappock

Article

Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

changes cysteine-357 to tyrosine

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK274

Plasmid

#71564

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 3)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1 promoter

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site in 35S promoter re…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA002

Plasmid

#48813

Purpose

Provides the AP3 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

AP3 promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site removed by substit…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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UAS-zw4 (egghead) ORF

Plasmid

#38764

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

zw4 (egh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCaShs-egghead (zw4)

Plasmid

#37400

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pSKB2-TRexR90D

Plasmid

#31350

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor (R90D)

Use

Tags

His

Expression

Bacterial

Mutation

changed Arginine 90 to Aspartic Acid

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K41E 3xFlag

Plasmid

#110105

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 41 to Glutamic acid (K41E)

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJYS1Ptac

Plasmid

#85545

Purpose

Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Tags

Expression

Bacterial

Mutation

Promoter

tac and unknown

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGene-TAP-GBP1

Plasmid

#138087

Purpose

Plasmid with TAP-GBP1 ORF

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Use

Tags

Tandem affinity purification (TAP) tag

Expression

Mammalian

Mutation

Promoter

Gal4-E1b

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMo130-TelR

Plasmid

#50799

Purpose

Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

Depositor

Kim Lee Chua

Article

Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

Insert

tellurite-resistance cassette

Use

Suicide vector

Tags

Expression

Mutation

Promoter

PCs12

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPSP

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-mEGFP

Plasmid

#214145

Purpose

Expresses the monomeric EGFP in eukaryotic cells

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

A207K L221K F223R

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLBM0010

Plasmid

#172816

Purpose

Level 2 module containing HSP21-GFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-GFP]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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