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We narrowed to 15,857 results for: cas9

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Showing: 1241 - 1260 of 15857 results

  4. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84239-plasmid-map-sequence-id-160811

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  6. CMV-hCas9-T2A-mStrawberry

    Plasmid
    #187224
    Purpose
    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.
    Depositor
    Jennifer Mitchell
    Publication
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Inserted T2A-mStrawberry at 3' terminal of M…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    187224-plasmid-map-sequence-id-367392

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  8. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid
    #101232
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G528A, V583A, E584A, D585A, N588A and Q926A
    Promoter
    T7
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101232-plasmid-map-sequence-id-197212

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  12. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid
    #181751
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181751-plasmid-map-sequence-id-353935

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  18. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid
    #73499
    Purpose
    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73499-plasmid-map-sequence-id-364405

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  19. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Feng Zhang
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68336-plasmid-map-sequence-id-119558

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  20. pRR1

    Plasmid
    #166096
    Purpose
    Gal inducible expression of Cas9 WT
    Depositor
    Rebecca Burgess
    Insert
    WT spCas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166096-plasmid-map-sequence-id-330354

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Showing: 1241 - 1260 of 15857 results