We narrowed to 5,067 results for: codon optimized

1. pX330-Flag-wtSpCas9 (without sgRNA)

   Plasmid

   #92353

   Purpose

   Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-wild-type SpCas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTO149_RFP_CauNEO

   Plasmid

   #177277

   Purpose

   Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   RFP, CauNEO
   

   Use

   Tags

   ENO1-RFP

   Expression

   Bacterial

   Mutation

   All CUG Leucine codons were replaced with the syn…

   Promoter

   AgTEF2

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMYO127TAG-SepT

   Plasmid

   #34625

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   Myoglobin-127TAG
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   codon-optimized + an amber stop codon was introdu…

   Promoter

   Ipp

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dm_λHA-FLuc0_CSC

   Plasmid

   #210589

   Purpose

   Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
   

   Use

   Luciferase

   Tags

   lambda HA

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH849-gst-optec

   Plasmid

   #89590

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmr101A-cPr-mNeonGreen (KmR)

   Plasmid

   #177209

   Purpose

   Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Yeast codon optimized

   Promoter

   Synthetic constitutive promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-L1PA1CH_blast rescue

   Plasmid

   #69611

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Use

   Tags

   blast rescue cassette

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBHM2641

   Plasmid

   #229525

   Purpose

   Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   CRISPR

   Tags

   t2a-mNeonGreen-AID*

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   TEF1

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBHM2647

    Plasmid

    #229989

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBHM2648

    Plasmid

    #229990

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBHM2649

    Plasmid

    #229991

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p426TEF-LptTPS-A

    Plasmid

    #89468

    Purpose

    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production

    Depositor

    Jing-Ke Weng

    Insert

    LptTPS-A
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    Promoter

    pBR322 origin of replication for propagation in E…

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKMYT1

    Plasmid

    #39061

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKMYT1 (PKMYT1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. SPR

    Plasmid

    #39161

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. PBRM1

    Plasmid

    #39013

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. PBRM1

    Plasmid

    #39027

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    polybromo 1 [isoform a] (PBRM1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only