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Showing: 1241 - 1260 of 5015 results

  1. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  3. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

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  5. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  7. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89590-plasmid-map-sequence-id-206038

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  8. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  11. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Use
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  12. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  13. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  14. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  15. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  16. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Use
    Tags
    none
    Expression
    Yeast
    Mutation
    Promoter
    pBR322 origin of replication for propagation in E…
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  17. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  18. pHH0103 NEDD4 WW domain #3

    Plasmid
    #104210
    Purpose
    Bacterial expression of WW domain #3 from NEDD4
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4 WW domain #3 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104210-plasmid-map-sequence-id-204199

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  19. pHH0103 NEDD4 WW domain #1

    Plasmid
    #104208
    Purpose
    Bacterial expression of WW domain #1 from NEDD4
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4 WW domain #1 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104208-plasmid-map-sequence-id-204367

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  20. pHH0103 NEDD4 WW domain #4

    Plasmid
    #104211
    Purpose
    Bacterial expression of WW domain #4 from NEDD4
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4 WW domain #4 (NEDD4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104211-plasmid-map-sequence-id-201499

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Showing: 1241 - 1260 of 5015 results