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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
3xFLAG-dCas9/pMSCVhyg

Plasmid

#134323

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-URH49 WT

Plasmid

#110127

Purpose

Expresses Flag-tagged human URH49 (DDX39A)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

April 6, 2019

Availability

Academic Institutions and Nonprofits only

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p4489 Flag-betaTrCP

Plasmid

#10865

Depositor

Peter Howley

Article

Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

Insert

betaTrCP (BTRC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Use

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Blast

Plasmid

#207717

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Use

Tags

Flag-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-3xFLAG-V5

Plasmid

#87063

Purpose

Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Flag-SREBP2

Plasmid

#134284

Purpose

Expresses Flag-tagged SREBP2 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-hPAX7 (#348)

Plasmid

#78337

Purpose

Overexpression of human PAX7 ORF with a N-terminal FLAG tag

Depositor

Matthew Alexander, Louis Kunkel

Insert

PAX7 (PAX7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag DLC1

Plasmid

#24265

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1

Plasmid

#62906

Purpose

Expresses wildtype IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p50 cFlag pcDNA3

Plasmid

#20018

Depositor

Stephen Smale

Insert

Nfkb1 (Nfkb1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Truncated at the p105 ligation site. It codes for…

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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Brm WT-Flag pShuttle

Plasmid

#122105

Purpose

Expression of full length Brm in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

BRM (SMARCA2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-GFP_PDGFA

Plasmid

#227949

Purpose

Co-localisation experiment

Depositor

Alessandro Ori

Insert

PDGFA (PDGFA Human)

Use

Lentiviral

Tags

3xFLAG-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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