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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

Plasmid

#238374

Purpose

Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting cmlc2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2987 [pNP2922 - ISCro4 TBL3 (WT) + ISCro4 Recombinase]

Plasmid

#247256

Purpose

Express ISCro4 recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2922 - ISCro4 bRNA35-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL3)

Expression

Mammalian

Mutation

C60U, A61G, U73C, dU86, A87G, dU88

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p221a-GUS

Plasmid

#71263

Purpose

Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Beta-glucuronidase

Use

Gateway

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGW03 AAV-TBG-Cre-sgRNA

Plasmid

#192163

Purpose

AAV-TBG-Cre-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PMP34-mCherry-EGFP-Tet-On

Plasmid

#109017

Purpose

Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

PMP34 (SLC25A17 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST-Constitutively Active AMPK

Plasmid

#139843

Purpose

Lentiviral expression of GST-tagged Constitutively Active rat AMPK

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Adeno EA

Plasmid

#64071

Purpose

Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Insert

U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Expression

Mammalian

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

Plasmid

#139844

Purpose

Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK-Kinase-dead (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Mutation

K47R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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hGAPDH-rLuc

Plasmid

#82479

Purpose

reporter of GAPDH expression

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

GAPDH promoter (GAPDH Human)

Use

Luciferase

Expression

Bacterial

Promoter

hGAPDH

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLux01

Plasmid

#78281

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3-deltaTM

Plasmid

#109025

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-deltaTM (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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