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Plasmid#206070Purposeexpression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationD491APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1594
Plasmid#143827PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF197 (ZNF197 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1593
Plasmid#142809PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF197 (ZNF197 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0220
Plasmid#142564PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP4 (FOXP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0222
Plasmid#142566PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP4 (FOXP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Alpha2 delta-1 V6
Plasmid#89894PurposeMammalian expression of Alpha2 delta-1 V6DepositorInsertCacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationAmino acids L943V, E944V, A945V, E947V, M948V are…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0221
Plasmid#142565PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP4 (FOXP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MR1-COMP5AP-AviTag-9xHis
Plasmid#157392PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertMR1 (MR1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201819PurposeA plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoterDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseTagsExpressionMammalianMutationPromoterpOTTC1751Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rat Alpha2 delta-1 pMT2
Plasmid#58726Purposeexpression of rat alpha2 delta-1 in mammalian cellsDepositorInsertalpha2 delta-1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MR1-Fc(DAPA)-AviTag-6xHis
Plasmid#156828PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertMR1 (MR1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP
Plasmid#127090PurposeCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activationDepositorHas ServiceAAV PHP.eBInsertChannelrhodopsin-2
UseAAVTagseYFP (C terminal on insert)ExpressionMammalianMutationHumanized ChR2 gene with histidine 134 changed to…PromoterCAGAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP
Plasmid#47633PurposeAn AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsiRFPExpressionMammalianMutationH134RPromoterEF1aAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65429PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201820PurposeAn adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imagingDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseAAVTagsExpressionMutationPromoterSYN1Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI AscI MR1 res
Plasmid#214752Purposeexpresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cellsDepositorInsertMHC class I-related protein 1 (MR1 Human)
UseTagsIRES eGFPExpressionMammalianMutationsilent mutations to prevent editing by CRISPR/Cas…PromoterCMVAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201821PurposeAn adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PETDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseAAVTagsExpressionMutationPromoterSYN1Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only