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We narrowed to 6,915 results for: tac

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Showing: 1241 - 1260 of 6915 results

  8. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  9. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  12. (SUMO)5-(SIM)10

    Plasmid
    #126923
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Expression
    Bacterial
    Mutation
    internal BamHI site between (SUMO)5 and (SIM)10
    Promoter
    tac
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126923-plasmid-map-sequence-id-255302

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Showing: 1241 - 1260 of 6915 results