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p252 pPr-CREM/CMV-STOP-luc

Plasmid

#8406

Purpose

Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin promoter, CMV

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 1-225 (#431)

Plasmid

#99927

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-225

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL VV458/460PP

Plasmid

#61368

Purpose

Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with VV458/460P mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P and V460to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSALECT-Efnb2

Plasmid

#59707

Purpose

pSALECT with Efnb2 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Efnb2 (Efnb2 Mouse)

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pSR04

Plasmid

#69151

Purpose

read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

eef-1A.1 (eft-3) and rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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HOXB4-FLAG-WG-SP65

Plasmid

#21087

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB4 (HOXB4 Human)

Use

In vitro t/t

Mutation

W to G mutation in YPWM Pbx1 interaction motif, p…

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only