We narrowed to 2,383 results for: LRE

1. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

   Plasmid

   #188704

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   NFATC2 sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYCDuet_D27NDHFR_WTTS

   Plasmid

   #91244

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D27N

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2_(-3kb)nrg1_typeII:GFP-CAAX

   Plasmid

   #199394

   Purpose

   pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   (-3kb)nrg1 type II
   GFP-CAAX
   afp_3'_UTR
   

   Use

   Tol2 gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101107

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2-cryR;clecl14aCE1-P1Egfp

   Plasmid

   #90157

   Purpose

   contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   clec14aE1P1Egfp
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXJ40 GADD34 (Delta Pest1)

   Plasmid

   #78096

   Purpose

   mammalian expression of GADD34 Delta Pest1

   Depositor

   Shirish Shenolikar

   Article

   Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

   Insert

   GADD34 (PPP1R15A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deletion of PEST1

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-mSNX27 RNAi-resistant

   Plasmid

   #163620

   Purpose

   mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   mouse SNX27 RNAi-resistant (Snx27 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RNAi resistance

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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