Addgene

We narrowed to 151,085 results for: Des

Showing: 2521 - 2560 of 151085 results

Results per page:

Sort results by:

p8.9 NdeltaSB D185K/D186L

Plasmid

#132931

Purpose

p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag and pol D185K/D186L

Use

Lentiviral

Tags

Expression

Mutation

D185K/D186L

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Human ICAM-3

Plasmid

#8857

Depositor

Timothy Springer

Article

de Fougerolles et al J Exp Med. 1992 Jan 1. 175(1):185-90.

Insert

ICAM-3 (ICAM3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only
pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-CRKL-Y198FY207F

Plasmid

#32506

Depositor

John Groffen, Nora Heisterkamp

Article

de Jong et al Oncogene. 1997 Feb 6;14(5):507-13.

Insert

CRKL (CRKL Human)

Use

Tags

Expression

Mammalian

Mutation

Y207F and Y198F

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221T7RNAP

Plasmid

#167974

Purpose

Expresses T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

T7RNAP (T7p07 Synthetic)

Use

Tags

Expression

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Use

Tags

T7RNAP and TrxA

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

qacA

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-BCL9

Plasmid

#113759

Purpose

For the expression of FLAG-BCL9 in mammalian cells

Depositor

Mariann Bienz

Article

de la Roche et al BMC Cancer. 2008 Jul 15;8:199. doi: 10.1

Insert

BCL9 (BCL9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVDM1001

Plasmid

#134660

Purpose

CRISPR delivery and repair template delivery vector

Depositor

Willem van Schaik

Article

de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

Insert

CRISPR guide RNA array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221rAPOBEC1-T7RNAP

Plasmid

#167979

Purpose

Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

rAPOBEC1-T7RNAP (T7p07 Synthetic)

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-NRBP-HA

Plasmid

#32504

Depositor

John Groffen, Nora Heisterkamp

Article

De Langhe et al Int J Mol Med. 2002 May;9(5):451-9.

Insert

NRBP (NRBP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221MBPc-AID-T7RNAP

Plasmid

#167975

Purpose

Expresses fusion protein MBPc-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

MBPc-AID-T7RNAP

Use

Tags

MBPc and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T Crkl SH3SH3

Plasmid

#36403

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl tandem SH3 domains (CRKL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pES002 (pTet-qacR wt)

Plasmid

#69031

Purpose

wild-type qacR downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Use

Tags

6xHis - TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T Crkl SH2

Plasmid

#36402

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl SH2 domain (CRKL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac1;2

Plasmid

#47765

Purpose

expresses AmTrac1;2 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-LS

Plasmid

#47767

Purpose

expresses AmTrac-LS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-LS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Use

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-AmTrac-100

Plasmid

#47771

Purpose

expresses AmTrac-100 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-100

Use

Tags

mcpGFP

Expression

Yeast

Mutation

A141E, T464D

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-meptrac

Plasmid

#47768

Purpose

expresses MepTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

meptrac

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-IS

Plasmid

#47766

Purpose

expresses AmTrac-IS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-GS

Plasmid

#47769

Purpose

expresses AmTrac-GS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-GS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pLX301

Plasmid

#25895

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304

Plasmid

#25890

Purpose

3rd generation lentiviral vector

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX302

Plasmid

#25896

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2-HA-RAPGEF2(S1244A/S1248A)

Plasmid

#110163

Purpose

Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S1244A/S1248A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Plenti-ARE-HSVTK-luc

Plasmid

#161789

Purpose

Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

Depositor

Hans-Guido Wendel

Article

Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

Inserts

Luciferase-T2A-HSV-TK
mCherry P2A blasticidin

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EF1a and Murine Gclc ARE

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20-α-Syn

Plasmid

#92200

Purpose

Retroviral overexpression vector (doxycycline-inducible) for α-Syn

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

SNCA (SNCA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-RAB11a KI

Plasmid

#131499

Purpose

Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rab11a, Rab11a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Cplx1-GFP KI

Plasmid

#131475

Purpose

Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2521 - 2560 of 151085 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests