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Showing: 2521 - 2560 of 3894 results

  1. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid
    #170254
    Purpose
    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    A662C, N664C, V667L
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170254-plasmid-map-sequence-id-334374

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  2. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  5. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid
    #170236
    Purpose
    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CHUK (CHUK Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    S176E, S180E
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170236-plasmid-map-sequence-id-334484

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  13. pJB48

    Plasmid
    #226307
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S CsoS2 MR1-6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226307-plasmid-map-sequence-id-449919

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  14. pJB47

    Plasmid
    #226306
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S CsoS2 MR1-5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226306-plasmid-map-sequence-id-449918

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  15. pJB46

    Plasmid
    #226305
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S CsoS2 MR1-4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S, C351S, C369S, C467S, C485S
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226305-plasmid-map-sequence-id-449917

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  16. pAGH42

    Plasmid
    #107249
    Purpose
    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp
    Use
    Suicide vector for destined for double homologous…
    Tags
    mGFPmut3
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter of sll0617
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107249-plasmid-map-sequence-id-207556

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  18. pWZ699

    Plasmid
    #208117
    Purpose
    Payload yeast assembly vector with TK-mActb on backbone
    Depositor
    Jef Boeke
    Article
    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-
    Type
    Empty backbone
    Use
    Synthetic Biology; Copy number inducible
    Tags
    Expression
    Bacterial, Mammalian, and Y…
    Mutation
    Promoter
    Human PGK1 for thymidine kinase
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208117-plasmid-map-sequence-id-409744

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  19. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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  20. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  25. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pHBJT034

    Plasmid
    #225180
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225180-plasmid-map-sequence-id-444744

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  28. pSM046

    Plasmid
    #217400
    Purpose
    KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc
    Depositor
    Brian Pfleger
    Article
    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:
    Insert
    BTE2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PTrc
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    217400-plasmid-map-sequence-id-434131

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  32. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  36. pDH3

    Plasmid
    #83789
    Purpose
    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    CFP
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    F64L, Y66W, N146I, M153T, V163A, N164H mutations …
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83789-plasmid-map-sequence-id-160016

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  37. pENT L1-Kozak-ERK-KTR-L2

    Plasmid
    #186353
    Purpose
    Entry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Patrick Lemaire
    Insert
    ERK Kinase Translocation Reporter (MAPK1 Synthetic, Human)
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186353-plasmid-map-sequence-id-373419

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Showing: 2521 - 2560 of 3894 results