We narrowed to 2,903 results for: BFP

1. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

   Plasmid

   #200503

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

   Plasmid

   #200505

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

   Plasmid

   #202826

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

   Plasmid

   #202825

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199450

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199453

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

   Plasmid

   #195501

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

   Plasmid

   #178871

   Purpose

   Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

   Plasmid

   #167921

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

    Plasmid

    #178870

    Purpose

    Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

    Plasmid

    #139237

    Purpose

    mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

    Plasmid

    #139234

    Purpose

    mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

    Plasmid

    #139233

    Purpose

    mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

    Plasmid

    #139232

    Purpose

    mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

    Plasmid

    #139231

    Purpose

    mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

    Plasmid

    #128764

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #3 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

    Plasmid

    #128763

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #2 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

    Plasmid

    #128762

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #1 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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