Addgene

We narrowed to 3,177 results for: Ubc

Showing: 1261 - 1280 of 3177 results

Results per page:

Sort results by:

FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SBFP2-anti-AlfaTag nanobody

Plasmid

#171826

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

SBFP2-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_cAMPFIRE-L

Plasmid

#182283

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_cAMPFIRE-L-R279E

Plasmid

#182282

Purpose

Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-ER-mTagBFP2

Plasmid

#216127

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-NLS-miRFP670nano-miRFP670

Plasmid

#216131

Purpose

Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-SpiCee-NLS

Plasmid

#140900

Purpose

mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NLS

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-PI4KAC1001(D1957A)

Plasmid

#139312

Purpose

Recruitable PI4KA kinase-dead catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Use

Tags

Expression

Mammalian

Mutation

D1957A, G1986D

Promoter

Available since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-ATXN1-52Q

Plasmid

#32492

Depositor

Huda Zoghbi

Article

Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

insert contains a stretch of 52 Glutamines and no…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pCX-SpiCee-NES

Plasmid

#140901

Purpose

mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-NES

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-PI4KAC1001

Plasmid

#139311

Purpose

Recruitable PI4KA catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Use

Tags

Expression

Mammalian

Mutation

G1986D

Promoter

Available since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-mAmetrine-CAAX

Plasmid

#216132

Purpose

Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-mAmetrine-CAAX-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-NTOM-SpiCee

Plasmid

#140902

Purpose

mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

NTOM-SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge