Addgene

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pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Tags

Expression

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSRW1JZ

Plasmid

#129528

Purpose

C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

Depositor

Noelle L'Etoile

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-dPCI-HA

Plasmid

#139847

Purpose

Lentiviral expression of human DDRGK1-dPCI-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

d229-314

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS10

Plasmid

#113956

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution…

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS1

Plasmid

#210633

Purpose

Luciferase experiment for TAL1 TSS1

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS1 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS2

Plasmid

#210634

Purpose

Luciferase experiment for TAL1 TSS2

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS2 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS4

Plasmid

#210635

Purpose

Luciferase experiment for TAL1 TSS4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS4 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS5

Plasmid

#210636

Purpose

Luciferase experiment for TAL1 TSS5

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS5 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

Plasmid

#113952

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, I132S and S212N su…

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-deltaTM

Plasmid

#109021

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-deltaTM (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(K211TAG)

Plasmid

#182660

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K211TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_S212N

Plasmid

#113951

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-dTM-HA

Plasmid

#139846

Purpose

Lentiviral expression of human DDRGK1-dTM-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

d1-27

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K267R-HA

Plasmid

#139848

Purpose

Lentiviral expression of human DDRGK1-K267R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

K267R

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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J23110-mRFP1-331Bb

Plasmid

#78277

Purpose

mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23110-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-ATP5O

Plasmid

#139842

Purpose

Lentiviral expression of human ATP5O

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

ATP5O (ATP5PO Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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