We narrowed to 1,758 results for: nif

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Sar1b-mOrange2

Plasmid

#166899

Purpose

mammalian expression of Sar1b tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mOrange2

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Plasmid

#222992

Purpose

Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ823

Plasmid

#39313

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (lin2744 Listeria innocua)

Use

CRISPR

Tags

MBP and thioredoxin-His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
Fra-1

Plasmid

#187906

Purpose

Expresses Fra-1 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra1 (Fosl1 Rat)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKEW106-MbCas12a-58069

Plasmid

#115653

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

Plasmid

#91861

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R0179A/K1080A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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JAB3603

Plasmid

#190422

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2493

Plasmid

#190375

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt

Expression

Plant

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2191

Plasmid

#190355

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_E299A

Plasmid

#91855

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) E299A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

E299A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pKEW107-MbCas12a-33362

Plasmid

#115654

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBA1018

Plasmid

#189587

Purpose

T4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T4wtGT7 Deletion Locus 250bp Homology Arms

Use

Synthetic Biology

Expression

Bacterial

Mutation

Encodes for a full deletion of T4 from 52.1 (part…

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1087A

Plasmid

#91864

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1087A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1087A

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

Plasmid

#39300

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S16A, R17A, H18A, D19A, W23A and N25A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only