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pCOLA-Spec-EV

Plasmid

#212095

Purpose

(Empty Backbone) pCOLA-Spec Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-D77

Plasmid

#18896

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-D77 (UBC Human)

Expression

Bacterial

Mutation

Aspartate residue is added to the C-terminus of t…

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIBB-mEGFP-IRES-puro2b

Plasmid

#26462

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pIBB

Tags

mEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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SRpHi4

Plasmid

#102544

Purpose

Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

EYFP-EGF

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCytERM-BDFP1.6-N1

Plasmid

#197179

Purpose

Expresses the fusion protein of CytERM-BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-BDFP1.6

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 (1-102)

Plasmid

#210427

Purpose

Bacterial expression of MBP-PB1(1-102) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-102) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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