161,152 results

1. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

   Plasmid

   #163697

   Purpose

   Expresses c-terminal half of TurboID on surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

   Plasmid

   #219828

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TFORF0574

   Plasmid

   #143714

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXA13 (HOXA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-K-SPOTIT

   Plasmid

   #191488

   Purpose

   Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   K-SPOTIT
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL2680

   Plasmid

   #85581

   Purpose

   Replicating base vector for constructing cpf1/CRISPR editing plasmids

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMP597

   Plasmid

   #134999

   Purpose

   Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV BARK p2A mCherry

   Plasmid

   #117689

   Purpose

   CMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs peptide
   

   Use

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPSup

   Plasmid

   #149416

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV BARK D110A p2A mCherry

    Plasmid

    #117690

    Purpose

    CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    D110A

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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