165,385 results

1. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR'-NRSF-CITE-GFP

   Plasmid

   #21310

   Depositor

   Jay Nadeau

   Article

   Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

   Insert

   neuron restrictive silencer factor (Rest Mouse)
   

   Use

   Lentiviral

   Tags

   GFP (bicistronic with CITE/IRES)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Oct. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-3'3xFlag

   Plasmid

   #208616

   Purpose

   Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HA-JMJD2B

   Plasmid

   #24181

   Purpose

   Mammalian expression of human JMJD2B with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2B (KDM4B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

   Plasmid

   #224435

   Purpose

   AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mScarlet-KASH
   U6-gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-hIFITM3

   Plasmid

   #58397

   Purpose

   Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWKS2

   Plasmid

   #139410

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   GFP S65T
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P2(GST-SNAP-Shp2tSH2)

    Plasmid

    #177907

    Purpose

    Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)
    

    Tags

    GST-PreScission cut site-SNAP tag

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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