We narrowed to 81,735 results for: TRI

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

Plasmid

#166010

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PA-DAD-wt

Plasmid

#53095

Purpose

Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

LOV(wt)-6aa-DAD

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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BII-BCA-Prom-ASCL2

Plasmid

#133394

Purpose

Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

destabilized tdTomato-NLS and destabilized NLS-GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free) drives expressi…

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/38]

Plasmid

#206777

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

Plasmid

#166011

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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