162,717 results

1. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

   Plasmid

   #111745

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q36 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HT PTP-RN

   Plasmid

   #24056

   Depositor

   Frederick Stanley

   Insert

   PTPRN (PTPRN Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKrox24(MapErk)Luc

   Plasmid

   #200112

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence in minimal p…

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSA3_His

   Plasmid

   #202765

   Purpose

   human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA3
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT2-HRP

   Plasmid

   #44021

   Depositor

   Dane Wittrup

   Article

   Lipovsek et al Chem Biol. 2007 Oct;14(10):1176-85.

   Insert

   HRP
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. mPA-GFP-CD81-10

   Plasmid

   #57124

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

   Plasmid

   #204621

   Purpose

   Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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