We narrowed to 818 results for: VILL;

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

   Plasmid

   #176085

   Purpose

   EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT2 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

   Plasmid

   #176074

   Purpose

   EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

   Plasmid

   #216478

   Purpose

   lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDY0738 pCAG-NLS-SscINT

   Plasmid

   #179113

   Purpose

   SscINT expression vector

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SscINT
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0739 pCAG-NLS-SacINT

   Plasmid

   #179114

   Purpose

   SacINT expression vector

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SacINT
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-AUP1-FLAG-DCUE

   Plasmid

   #185339

   Purpose

   Assessing domain requirements of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 293-337 corresponding to the CUE d…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0312 PhiBT Integrase

    Plasmid

    #179111

    Purpose

    PhiBT Integrase expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    PhiBT Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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