We narrowed to 31,362 results for: ica

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pET-51b(+)-Caprola_02

Plasmid

#194663

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_02 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_02

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_03

Plasmid

#194664

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_03 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_03

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

Plasmid

#205704

Purpose

Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola5-EGFP

Expression

Insect

Promoter

20 Gal4

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45

Plasmid

#208796

Purpose

Xenopus laevis FLAG5-His10-TEV-Cdc45

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

Cdc45 (cdc45.L Frog)

Tags

FLAG and His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-WM

Plasmid

#208798

Purpose

Xenopus laevis His6-Myc-DONSON (W234L, M463T)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

W234L; M463T

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_LF_WPRE

Plasmid

#209766

Purpose

AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_LF

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA-Flag-uMASS_1_WPRE

Plasmid

#209760

Purpose

AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HT121_Fsyn_QuasAr6a(Q3_codon)_citrine

Plasmid

#208606

Purpose

Expressing QuasAr6a_Citrine with an alternate codon

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a

Use

Lentiviral

Tags

Citrine

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Expression

Bacterial

Promoter

hns promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Expression

Bacterial

Promoter

hns promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV1:T35s

Plasmid

#164738

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr6g043230.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV9:T35s

Plasmid

#164742

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr4g116360.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV10:T35s

Plasmid

#164739

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr8g446520.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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hsa-cdk2 3'UTR sicheck2

Plasmid

#121125

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-cdk2 3'UTR (CDK2 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45 (delta 154–164)

Plasmid

#126879

Purpose

Expresses crystallisation construct of human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

Human Cdc45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Mutation

deletion amino acid 154-164

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a V52A

Plasmid

#90050

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT/V52A

Expression

Bacterial

Mutation

V52A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a Q55A

Plasmid

#90052

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA/Q55A

Expression

Bacterial

Mutation

Q55A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a Q55I

Plasmid

#90053

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC/Q55I

Expression

Bacterial

Mutation

Q55I

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CC1

Plasmid

#63713

Purpose

Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

CC1

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4-rB90

Plasmid

#18959

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican C-terminus (Bcan Rat)

Expression

Mammalian

Mutation

Rat brevican C-terminus: Signal peptide aa 1-22 …

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf46

Plasmid

#12734

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf46

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only