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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SIV_Gag_Pol

Plasmid

#236243

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Gag and Pol

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

Plasmid

#195048

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

Plasmid

#195047

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

Plasmid

#195046

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

pADH2, pGPD, and pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1617

Plasmid

#29279

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple146 (NTSR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades…

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1712

Plasmid

#29297

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX_DSB_Spectrum-V1

Plasmid

#192474

Purpose

Lentiviral DSB Spectrum V1 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMC037-HisMS2_PLP_pac

Plasmid

#128233

Purpose

Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX_DSB_Spectrum-V2

Plasmid

#192475

Purpose

Lentiviral DSB Spectrum V2 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-TT-PA-CRE

Plasmid

#179584

Purpose

ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

PA-MAG-CRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Use

Tags

6xHis - TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEM-mcpGFP

Plasmid

#47442

Purpose

contains modified circularly permutated EGFP (mcpGFP)

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

mcpGFP

Use

Tags

Expression

Bacterial

Mutation

M154K, V164A, S176G, D181Y, T204V, A207K, V190I

Promoter

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pES005 (pTet-qacR wt/pQacA-deGFP)

Plasmid

#69034

Purpose

reporter with wild-type qacR on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

pQacA and pTet

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Use

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pES004 (pTet-qacR 5)

Plasmid

#69033

Purpose

qacR 5 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pTet

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl SH2SH3

Plasmid

#36404

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl SH2 and N-terminal SH3 domains (CRKL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-MEKDD

Plasmid

#31202

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S218D S222D

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Dlg4-GFP KI

Plasmid

#131477

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-GFP-IKBalpha-mut (super repressor)

Plasmid

#15264

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-GFP-IKBalpha-wt

Plasmid

#15263

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti GW V5 Eco/Dam hum LaminB1

Plasmid

#182671

Purpose

Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.

Depositor

Bas van Steensel

Article

Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do

Insert

Lamin B1 (LMNB1 Human)

Use

Lentiviral

Tags

E. Coli Dam and V5

Expression

Mammalian

Mutation

Promoter

HSP

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

Plasmid

#176836

Purpose

Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP

Use

Unspecified

Tags

Expression

Mutation

See Depositor Comments Below

Promoter

Tet operator (tetracycline inducible expression)

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Camk2a KI

Plasmid

#131484

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSV39-ugi

Plasmid

#186556

Purpose

Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

ugi

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Grin1 KI

Plasmid

#131485

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20-Tau

Plasmid

#92201

Purpose

Retroviral overexpression vector (doxycycline-inducible) for Tau

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSV39-dddAI

Plasmid

#186557

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-CACNA1A KI

Plasmid

#131480

Purpose

Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE mEos3.2-Camk2a KI

Plasmid

#131493

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and mEos3.2 donor (Camk2a, Camk2a Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
K84M kinase-dead Nuak1-3xflag

Plasmid

#97219

Purpose

Plasmid expressing kinase-dead mutant Nuak1

Depositor

Huda Zoghbi

Article

Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

Insert

Nuak1 (NUAK1 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Kinase-dead K84M

Promoter

T7

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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