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Plasmid#127314PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-AlstR-IRES2-EGFP
Plasmid#14895DepositorInsertAlstR (AstA-R1 Fly)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HC078 (3xHA-Atg13)
Plasmid#59544PurposeExpresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
UseTags3xHAExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB8 (murine) HIS-tag pET
Plasmid#8540DepositorInsertHOXB8 (Hoxb8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationstarts at amino acid #2 (Ser)PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
Plasmid#47542Purposeengineered receptor improves heteromeric receptor expression and sensitivity to IVM.DepositorInsertGlutamate-gated chloride channels
UseTagsExpressionMammalianMutationretention motif in ER (starting at R340) changed …PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pENTR1A hu_ncOGT WT (open N-term)
Plasmid#155197PurposeEntry vector encoding non-tagged O-GlcNAc Transferase with open N-terminusDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway cloningTagsExpressionMutationWT OGT with no start codon, in-frame with gateway…PromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127315PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 d80 pET21a+
Plasmid#173045PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
UseTags6xHis tagExpressionBacterialMutationwith N-terminal 80 amino acids deletionPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXB2 (human) HIS-tag pET
Plasmid#8522DepositorInsertHOXB2 (HOXB2 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at AA #2; makes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N
Plasmid#22018DepositorInsertmVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutation T17N.PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBI101-pGC1(D1)-GUS
Plasmid#49513PurposeExpresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plantDepositorInsertPromoter of GC1(D1) (AT1G22690 Mustard Weed)
UsePlant expressionTagsbeta-glucuronidaseExpressionMutationPromoterGC1 (At1g22690), 1141 bp upstream of the transcri…Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVL94 - pOp4:VND7
Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOXC4 (human) MSCV
Plasmid#8546DepositorInsertHOXC4 (HOXC4 Human)
UseRetroviralTagsExpressionMammalianMutationContains improved ribosomal binding site and NcoI…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-DNAJA4CDS
Plasmid#41849DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseLuciferaseTagsrenilla luciferaseExpressionMutationStart codon of DNAJA4 removedPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG
Plasmid#127308PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST
Plasmid#127318PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT250_(pCA-G-intron(Neo)-tTA2)
Plasmid#36876DepositorInsertsGFP
tTA2
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDK480
Plasmid#134197PurposeMammalian expression of Flip in SKAP and IRES GFPDepositorInsertuntagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
UseTagsIRES-GFPExpressionMammalianMutationAlternate Start, G5 siRNA hardenedPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-Met-Eif4a1-tdiRFP670-PEST
Plasmid#122371PurposeTargeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.DepositorInsert2A-tdiRFP-PEST-2A with homology arms for Eif4a1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(LNL)
Plasmid#36907DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 HopU1
Plasmid#228395PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tagsDepositorInsertHopU1
UseGateway-compatible entry vectorTagsExpressionMutationAlanine inserted after start Methionine due to Nc…PromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQE-meGFP-DLC1
Plasmid#192048PurposePlasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFPDepositorInsertDLC1 (DLC1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1
Plasmid#220117PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoterDepositorInsertTadA8e, nSauriCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1
Plasmid#220120PurposeExpresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoterDepositorInsertMYL2, TadA, nSauriCas9
UseAAVTagsExpressionMutationPromoterMYL2Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSG068
Plasmid#214259PurposepJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaIDepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutation18 nucleotide 5' of the start codon (Shine-D…PromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF1
Plasmid#186765PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL464
Plasmid#69881Purposea dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORFDepositorInsertCIRL (Cirl Fly)
UsePhic31-integration vectorTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 800
Plasmid#131147PurposeTo investigate activity of a 800 bp region upstream of mouse Trib2.DepositorInsert-800 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 2.6
Plasmid#131151PurposeTo investigate activity of a 2.6 kb region upstream of mouse Trib2.DepositorInsert-2.6kb - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 T2P 500
Plasmid#131150PurposeTo investigate activity of a 500 bp region upstream of mouse Trib2.DepositorInsert-500 - 0 of mTrib2 upstream of gene transcriptional start site (Trib2 Mouse)
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only