We narrowed to 5,133 results for: codon optimized
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Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS
Plasmid#126203PurposeMammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIC3Nme
TagsFLAG/NLS and mCherryExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1149-pCSDest-AcrIIA4-mCherry-miR122BS
Plasmid#126205PurposeMammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIA4
TagsFLAG/NLS and mCherryExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR596Q-myc
Plasmid#107483PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotypeDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK605N-myc
Plasmid#107475PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF465L-myc
Plasmid#107469PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotypeDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR462E-myc
Plasmid#107489PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG582K-myc
Plasmid#107497PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI576K-myc
Plasmid#107496PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF556H-myc
Plasmid#107495PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F556H conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTP499K-myc
Plasmid#107494PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation P499K conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTE494Y-myc
Plasmid#107493PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E494Y conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK490L-myc
Plasmid#107492PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K490L conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL467D-myc
Plasmid#107490PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L467D with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTC473H-myc
Plasmid#107491PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation C473H conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTY410G-myc
Plasmid#107488PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y410G with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI379Q-myc
Plasmid#107485PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I379Q conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTV343T-myc
Plasmid#107484PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation V343T conffering gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF263C-myc
Plasmid#107482PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F263C with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTA173G-myc
Plasmid#107479PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A173G with approximately wildtype activity, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTF88H-myc
Plasmid#107478PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F88H conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR79V-myc
Plasmid#107477PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R79V conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR464Q-myc
Plasmid#107468PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R464Q approximately wildtype transport abilityDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTT448K-myc
Plasmid#107467PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T448K conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTT439M-myc
Plasmid#107466PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI425L-myc
Plasmid#107465PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expressionDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG384S-myc
Plasmid#107463PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS375L-myc
Plasmid#107462PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTA331T-myc
Plasmid#107461PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTA169D-myc
Plasmid#107460PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expressionDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 1
Plasmid#70651PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 2
Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_Triple
Plasmid#67733PurposeExpresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged histidine 303 to alanine, arginine 373 to…PromoterT7Available SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA3228
Plasmid#46856PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12DepositorInsertExoY K81M
UseLentiviralTagsFKBP E31G, R71G, K105E and MycPromoterTRE-TightAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3_nostop
Plasmid#149306PurposeGateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP6_nostop
Plasmid#149309PurposeGateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits