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pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Promoter

cpc operon

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
SRS-MTBD(26/19)ux, dimer, no myc

Plasmid

#22382

Depositor

Ron Vale

Article

Carter et al Science. 2008 Dec 12. 322(5908):1691-5.

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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XE95 mFZ6-CS2+

Plasmid

#16802

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ6-CS2+ (Fzd6 Mouse)

Use

Xenopus expression

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
XE105 XPKC alpha-myc-CS2+

Plasmid

#16745

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

XPKC-alpha-myc (prkca Frog)

Use

Xenopus expression

Tags

c-myc

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1_N-NLS

Plasmid

#221867

Purpose

Empty backbone for expressing GFP-fusion proteins in mammalian cells with a nuclear localization signal (NLS).

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG

Plasmid

#220537

Purpose

AAVS1-targeting backbone used as a filler plasmid to co-deliver during nucleofection with pAAV-Puro_siKD2.0 (Plasmid #220536) cloned with shRNAs

Depositor

Alessandro Bertero

Article

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Multi-Janus-Spacer-1x_pKG1126

Plasmid

#246375

Purpose

Multi-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9

Plasmid

#241827

Purpose

Expresses MMLVgag–dCas9 for producing RENDER-dCas9

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9

Use

CRISPR

Tags

FLAG, HA and TagBFP

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-AHR-shRNA2

Plasmid

#166908

Purpose

Lentivirus for inducible knockdown of human AHR

Depositor

Roland Friedel

Article

Halawani et al bioRxiv [Preprint]. 2024 Sep 14:2023.11.

Insert

AHR shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-eGFP

Plasmid

#242758

Purpose

Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-EGFP

Expression

Bacterial

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

Plasmid

#239237

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

Plasmid

#239240

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

Plasmid

#239242

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

Plasmid

#239258

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

Plasmid

#239259

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH

Plasmid

#239728

Purpose

Plasmid expressing mRuby2 and PuroR with the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH

Plasmid

#239729

Purpose

Plasmid expressing mRuby2 and PuroR with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2438_pGEEC534_pShip-CMV-mRuby2-P2A-PuroR-bGH

Plasmid

#239730

Purpose

Plasmid expressing mRuby2 and PuroR with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2439_pGEEC535_pShip-hPGK-mRuby2-P2A-PuroR-bGH

Plasmid

#239731

Purpose

Plasmid expressing mRuby2 and PuroR with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2441_pGEEC537_pShip-CAG-mRuby2-P2A-PuroR-bGH

Plasmid

#239733

Purpose

Plasmid expressing mRuby2 and PuroR with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2387_pPV2-mRuby2-P2A-PuroR

Plasmid

#239696

Purpose

BsaI Golden Gate assembly plasmid encoding the mRuby2-P2A-PuroR CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCARGO-RMCE

Plasmid

#233264

Purpose

Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Type

Empty backbone

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1022

Plasmid

#225593

Purpose

The backbone vector for rice genome editing is based on HkCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1023

Plasmid

#225594

Purpose

The backbone vector for rice genome editing is based on PrCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1024

Plasmid

#225595

Purpose

The Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1025

Plasmid

#225596

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1026

Plasmid

#225597

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a-R (D172R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225704

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mTurquoise

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a:ChrH

Plasmid

#225084

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Tags

His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a:ChrI

Plasmid

#225085

Purpose

Expresses anthrone-oxygenase like enzyme ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrI

Tags

His6 and His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK468 (BSD-P2A-mAID-dTAG)

Plasmid

#214394

Purpose

mAID-dTAG for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-mAID-dTAG

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK452 (BSD-P2A-dTAG-mClover)

Plasmid

#214382

Purpose

dTAG-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-dTAG-mClover

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28b(+)/ADK C77S

Plasmid

#201409

Purpose

Overexpresses Escherichia coli adenylate kinase C77S

Depositor

Gary Pielak

Article

Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

Insert

Escherichia coli adenylate kinase C77S (adk Escherichia coli)

Expression

Bacterial

Mutation

changed cysteine 77 to serine

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN BFP

Plasmid

#188644

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

BFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXC3cIfm

Plasmid

#193750

Purpose

Expresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLUX

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPINA7A2mTFP1(F)

Plasmid

#193774

Purpose

Expresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mTFP1

Use

Synthetic Biology

Expression

Bacterial

Promoter

PINA7 synthetic promoter carrying binding sites f…

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PL752-hGH-polyA-PGK-puroR

Plasmid

#191682

Purpose

Donor plasmid backbone PGK-puroR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM20-9S-S2 [rpoA]

Plasmid

#176217

Purpose

Expression of TRX-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

HIS and TRX

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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