We narrowed to 12,287 results for: BASE
-
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA12 (GNA12 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position A(133)FPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e1 sgRNA / hSpCas9
Plasmid#172830PurposeMammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e2 sgRNA / hSpCas9
Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 P742 miniABEMax V82G
Plasmid#135385PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )DepositorInsertnSaCas9 P742 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 Q731 miniABEMax V82G
Plasmid#135386PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )DepositorInsertnSaCas9 Q731 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 Q737 miniABEMax V82G
Plasmid#135387PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )DepositorInsertnSaCas9 Q737 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 S740 miniABEMax V82G
Plasmid#135388PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 )DepositorInsertnSaCas9 S740 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 T128 miniABEMax V82G
Plasmid#135389PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 )DepositorInsertnSaCas9 T128 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 D127 miniABEMax V82G
Plasmid#135366PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 )DepositorInsertnSaCas9 D127 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 D126 miniABEMax V82G
Plasmid#135367PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 )DepositorInsertnSaCas9 D126 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E739 miniABEMax V82G
Plasmid#135368PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 )DepositorInsertnSaCas9 E739 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E743 miniABEMax V82G
Plasmid#135369PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 )DepositorInsertnSaCas9 E743 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E745 miniABEMax V82G
Plasmid#135370PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 )DepositorInsertnSaCas9 E745 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 F733 miniABEMax V82G
Plasmid#135371PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 )DepositorInsertnSaCas9 F733 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N730 miniABEMax V82G
Plasmid#135372PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 )DepositorInsertnSaCas9 N730 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E125 miniABEMax V82G
Plasmid#135373PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 )DepositorInsertnSaCas9 E125 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only