We narrowed to 2,422 results for: NIG

1. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKCAG-H2BmCherry-OptoJNKi

   Plasmid

   #89741

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Optogenetics

   Tags

   Histone2B and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-HF1-PGK-Puro

   Plasmid

   #110857

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VQRRA-PGK-Puro

   Plasmid

   #110858

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75234

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75235

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-VRERRA-PGK-Puro

   Plasmid

   #110859

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-VQR-P2A-Puro

   Plasmid

   #110848

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-VRERRA-P2A-Puro

   Plasmid

   #110852

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBI121::chitGFP5:AQ

    Plasmid

    #240450

    Purpose

    Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.

    Depositor

    Christoph Plieth

    Article

    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

    Insert

    Fusion of Chitinase signal, smGFP5, and Aequorin
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    GFP5 for expression plants (PMID 9122158); Solubl…

    Promoter

    Cauliflower mosaic virus 35S promoter (GenBank AJ…

    Available since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

    Plasmid

    #223790

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    WIPI2d IDR (WIPI2 Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    aa 364-425 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

    Plasmid

    #223758

    Purpose

    Stable expression and inducible recruitment of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    WIPI2 IDR (WIPI2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-mEGFP

    Expression

    Mutation

    aa 364-425 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGenlenti_ATG13-WT

    Plasmid

    #223771

    Purpose

    Stable expression of protein in mammalian cell culture, rescue experiments

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

    Plasmid

    #223751

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    6xHis-TEV-mCherry

    Expression

    Bacterial

    Mutation

    K87A/K88A

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

    Plasmid

    #223724

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    FIP200-CTR (RB1CC1 Human)
    

    Use

    Tags

    6xHis-TEV-EGFP

    Expression

    Bacterial

    Mutation

    aa 1429-1591 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1_GST-LC3C

    Plasmid

    #223727

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-126 only, with Glycine exposed

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG_mCherry-ATG13 (WT)

    Plasmid

    #223735

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_ATG101

    Plasmid

    #223796

    Purpose

    Expression of recombinant protein for purification (co-expression with ATG13)

    Depositor

    Sascha Martens, James Hurley

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG101 (ATG101 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG_GST-TEV-WIPI1

    Plasmid

    #223798

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens, James Hurley

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    WIPI1 (WIPI1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

    Plasmid

    #226101

    Purpose

    HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SIFI degron helices 1 & 2 deleted (Δ61-112)

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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