We narrowed to 4,220 results for: PRS

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

Plasmid

#146213

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

Plasmid

#146215

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-E236Q (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

Plasmid

#146216

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-K135A (EDC3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PTPRS-COMP5AP-AviTag-9xHis

Plasmid

#157602

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTPRS (PTPRS Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-111 (MCSII)_D

Plasmid

#146105

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-111

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-111 (DDX6 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-173 (MCSII)_D

Plasmid

#146106

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-173

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-173 (DDX6 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-398 (MCSII)_D

Plasmid

#146107

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-398

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-398 (DDX6 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-263_GST-HsRCK_70-472_D

Plasmid

#146110

Purpose

Bacterial Expression of HsEDC3_192-508del231-263-HsRck_70-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-263-HsRck_70-472 (EDC3 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-263_GST-HsRCK_296-472_D

Plasmid

#146111

Purpose

Bacterial Expression of HsEDC3_192-508del231-263-HsRck_296-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-263-HsRck_296-472 (EDC3 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3 _192-508del231-254_GST-HsRCK_D

Plasmid

#146112

Purpose

Bacterial Expression of HsEDC3_192-508del231-254-HsRck

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-254-HsRck (EDC3 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190078

Purpose

express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus ferrumequinum)

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P

Plasmid

#147220

Purpose

Bacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

Plasmid

#182305

Purpose

E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH-Countdown insertion into GECO 1.2 calcium sensing domains

Tags

A modified TorA periplasmic targeting sequence pl…

Expression

Bacterial

Mutation

Spontaneous mutation of calmodulin's penulti…

Promoter

t7

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-254_GST-HsRCK_70-472_D

Plasmid

#146108

Purpose

Bacterial Expression of HsEDC3_192-508del231-254-HsRck_70-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-254-HsRck_70-472 (EDC3 Human)

Expression

Bacterial

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-C4A,C5A,C7A-GFP

Plasmid

#166838

Purpose

Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

C4A, C5A, C7A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

Plasmid

#68275

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4A) mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB1 Cterminal tail

Plasmid

#60400

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only