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pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pWT046a

Plasmid

#107897

Purpose

W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA3

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAID6

Plasmid

#136989

Purpose

Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

Depositor

Huimin Zhao

Publication

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Inserts

dLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4

Expression

Yeast

Promoter

ENO2, TDH3, TEF1, and TPI1

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-iRFP670-Tdtomato-GFP

Plasmid

#111817

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
Tdtomato
GFP

Use

Cloning intermediate

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Expression

Mammalian

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOD_A0103

Plasmid

#91000

Purpose

Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

CmYLCV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

Plasmid

#154829

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

Depositor

Lise Marie Grav

Publication

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

coEPO
coEPO

Use

Cre/Lox

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R272

Plasmid

#138221

Purpose

Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

Depositor

Baojun Wang

Publication

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTK645

Plasmid

#114711

Purpose

Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1700-2115)-RFP-Nano

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR eGFPCRE L1-R5

Plasmid

#43960

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

eGFPCre

Use

Multisite gateway entr clone

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pJB066

Plasmid

#98913

Purpose

Express FtsZ tagged with pAmCherry1 in E coli

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

pAmCherry1

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1510

Plasmid

#91036

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-PT2A-GFP-iRFP670-Tdtomato

Plasmid

#111813

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
iRFP670
Tdtomato

Use

Retroviral

Expression

Mammalian

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWT063a

Plasmid

#107908

Purpose

W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

TRESG-BE3

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR puroR R4-R3

Plasmid

#43965

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Available since

Sept. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR IRES L5-L4

Plasmid

#43964

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

IRES

Use

Multisite gateway entr clone

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR mCherry L1-R5

Plasmid

#43963

Depositor

Peter Hohenstein

Publication

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

mCherry

Use

Multisite gateway entr clone

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMOD_C3001

Plasmid

#91094

Purpose

Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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